Human PPP1CC (PP1C gamma) Knockout HeLa Cell Line

Cat. No.
CLCP-00350298
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HeLa
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Cervix
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Female

Target Information

Gene Name
PPP1CC
Gene ID
UniProt No.
Gene Function
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence Similarities
Belongs to the PPP phosphatase family. PP-1 subfamily.

Storage & Handling

Recommended Control
Human wild-type HeLa cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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