Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Sequence Similarities
Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains.
Gene Function
Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
Post-translational Modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
