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Human PLS3 (T Plastin) Knockout HEK293T Cell Lysate

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Cat. No.
CLCP-00350608
Product Size
1 Kit

Product Overview

Species
Human
Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Form
Liquid
Tissue
Kidney
Morphology
Epithelial
Gender
Female
Mycoplasma Free
Yes

Target Information

UniProt No.
P13797
Sequence Similarities
Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains.
Gene ID
5358
Gene Name
PLS3
Gene Function
Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
Post-translational Modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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