Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp deletion in exon 3 and 4 bp deletion in exon 3.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Sequence Similarities
Belongs to the H-rev107 family.
Gene Function
Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. N- and O-acylation activity is hardly detectable.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
