Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
HCT116
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Sequence Similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.Contains 1 PH domain.Contains 1 protein kinase domain.
Post-translational Modifications
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Storage & Handling
Recommended Control
Human wild-type HCT116 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
