Human PCNT (Pericentrin) Knockout HEK293T Cell Line

Cat. No.
CLCP-00350285
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 12
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Kidney
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Female

Target Information

Gene Name
PCNT
Gene ID
UniProt No.
Gene Function
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

Storage & Handling

Recommended Control
Human wild-type HEK293T cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

Online Inquiry