Human PAK4 Knockout HEK293T Cell Lysate

Cat. No.
CLCP-00350599
Product Size
1 Kit

Product Overview

Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, homozygous: 1 bp insertion in exon 4.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
Sanger Sequencing, WB

Cell Line Properties

Tissue
Kidney
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
PAK4
Gene ID
UniProt No.
Gene Function
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Post-translational Modifications
Autophosphorylated on serine residues when activated by CDC42/p21.Phosphorylated on tyrosine residues upon stimulation of FGFR2.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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