Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, homozygous: 1 bp insertion in exon 4.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
Sanger Sequencing, WB
Cell Line Properties
Target Information
Gene Function
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Post-translational Modifications
Autophosphorylated on serine residues when activated by CDC42/p21.Phosphorylated on tyrosine residues upon stimulation of FGFR2.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.