Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
Sanger Sequencing, WB
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.
Post-translational Modifications
Autophosphorylated on serine residues when activated by CDC42/p21. Phosphorylated on tyrosine residues upon stimulation of FGFR2.
Storage & Handling
Recommended Control
Human wild-type HEK293T cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
