Human MYL9 Knockout A-431 Cell Lysate

Cat. No.
CLCP-00350582
Product Size
1 Kit

Product Overview

Parental Cell Line
A431
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion, 38 bp deletion; Frameshift = 99.02%
Knockout Validation
Next Generation Sequencing (NGS)
Tested Applications
Next Generation Sequencing

Cell Line Properties

Tissue
Epidermoid carcinoma
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
MYL9
Gene ID
UniProt No.
Gene Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Sequence Similarities
Contains 3 EF-hand domains.
Post-translational Modifications
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

Online Inquiry