Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 7
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Sequence Similarities
Contains 1 FHA domain.Contains 16 K167R repeats.Contains 1 PP1-binding domain.
Post-translational Modifications
Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Storage & Handling
Recommended Control
Human wild-type HeLa cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
