Product Overview
Parental Cell Line
HCT116
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and insertion of the selection cassette in exon 9.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Sequence Similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain.
Post-translational Modifications
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
