Human MARK2 Knockout HCT116 Cell Line

Inquiry

Cat. No.

CLCP-00350246

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HCT116

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing

Cell Line Properties

Tissue

Colon carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

MARK2

Gene ID

2011

UniProt No.

Q7KZI7

Gene Function

Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.

Sequence Similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.Contains 1 KA1 (kinase-associated) domain.Contains 1 protein kinase domain.Contains 1 UBA domain.

Storage & Handling

Recommended Control

Human wild-type HCT116 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

McCoY5a + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.