Human LPIN1 Knockout A549 Cell Line

Cat. No.
CLCP-00350235
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Knockout Validation
Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Male

Target Information

Gene Name
LPIN1
Gene ID
UniProt No.
Gene Function
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
Sequence Similarities
Belongs to the lipin family.
Post-translational Modifications
Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid. Sumoylated.

Storage & Handling

Recommended Control
Human wild-type A549 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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