Human LGALS9 (Galectin 9/Gal-9) Knockout THP-1 Cell Lysate

Cat. No.
CLCP-00350566
Product Size
1 Kit

Product Overview

Species
Human
Parental Cell Line
THP-1
Biosafety Level
BSL-1
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift: 98%
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Form
Liquid
Tissue
Acute monocytic leukemia
Morphology
Monocytic
Gender
Male
Mycoplasma Free
Yes

Target Information

UniProt No.
Sequence Similarities
Contains 2 galectin domains.
Gene ID
Gene Name
LGALS9
Gene Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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