Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift: 98%
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Cell Line Properties
Tissue
Acute monocytic leukemia
Adherent / Suspension
Suspension
Target Information
Sequence Similarities
Contains 2 galectin domains.
Gene Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
Storage & Handling
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
