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Human LGALS9 (Galectin 9/Gal-9) Knockout A549 Cell Line

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Cat. No.
CLCP-00350223
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Species
Human
Parental Cell Line
A549
Biosafety Level
BSL-1
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Male

Target Information

UniProt No.
O00182
Sequence Similarities
Contains 2 galectin domains.
Gene ID
3965
Gene Name
LGALS9
Gene Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.

Storage & Handling

Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
Recommended Control
Human wild-type A549 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.

For Research Use Only. Not For Clinical Use.

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