Human LGALS3 Knockout HCT116 Cell Line

Inquiry

Cat. No.

CLCP-00350221

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HCT116

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue

Colon carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

LGALS3

Gene ID

3958

UniProt No.

P17931

Gene Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.

Sequence Similarities

Contains 1 galectin domain.

Storage & Handling

Recommended Control

Human wild-type HCT116 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

McCoY5a + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.