Product Overview
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift = 100%
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA.
Sequence Similarities
Belongs to the intermediate filament family.
Post-translational Modifications
Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
