Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 13 bp deletion; Frameshift = 99.9%
Knockout Validation
Immunocytochemistry (ICC), Next Generation Sequencing (NGS), Western Blot (WB)
Tested Applications
ICC, WB
Cell Line Properties
Tissue
Epidermoid carcinoma
Adherent / Suspension
Adherent
Target Information
Gene Function
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Sequence Similarities
Belongs to the intermediate filament family.
Storage & Handling
Recommended Control
Human wild-type A-431 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
