Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 4 and insertion of the selection cassette in exon 4.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Sequence Similarities
Belongs to the kinesin light chain family. Contains 6 TPR repeats.
Post-translational Modifications
Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
