Human IFIT2 Knockout A549 Cell Lysate

Cat. No.
CLCP-00350545
Product Size
1 Kit

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Gender
Male
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
IFIT2
Gene ID
UniProt No.
Gene Function
IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
Sequence Similarities
Belongs to the IFIT family. Contains 6 TPR repeats.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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