Human IFI35 Knockout A549 Cell Lysate

Cat. No.
CLCP-00350544
Product Size
1 Kit

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 3 and 1 bp insertion in exon 3.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Gender
Male
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
IFI35
Gene ID
UniProt No.
Gene Function
Acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines.
Sequence Similarities
Belongs to the NMI family.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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