Product Overview
Parental Cell Line
HEK293
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Sequence Similarities
Belongs to the small GTPase superfamily. Ras family.
Post-translational Modifications
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
