Human FSCN1 (Fascin) Knockout HCT116 Cell Pellet

Cat. No.
CLCP-00350757
Product Size
1 Kit

Product Overview

Parental Cell Line
HCT116
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and insertion of the selection cassette in exon 1.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Colon carcinoma
Morphology
Epithelial
Gender
Male

Target Information

Gene Name
FSCN1
Gene ID
UniProt No.
Gene Function
Organizes filamentous actin into bundles with a minimum of 4. 1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
Sequence Similarities
Belongs to the fascin family.
Post-translational Modifications
Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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