Human FER Knockout HeLa Cell Line

Cat. No.
CLCP-00350152
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HeLa
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 4
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Cervix
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Female

Target Information

Gene Name
FER
Gene ID
UniProt No.
Gene Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Sequence Similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.Contains 1 FCH domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.

Storage & Handling

Recommended Control
Human wild-type HeLa cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% DMSO, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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