Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
HCT116
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Sequence Similarities
Contains 1 FERM domain.
Post-translational Modifications
Phosphorylated by tyrosine-protein kinases.
Storage & Handling
Recommended Control
Human wild-type HCT116 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
