Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 6 and insertion of the selection cassette in exon 6.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Sequence Similarities
Contains 1 FERM domain.
Post-translational Modifications
Phosphorylated by tyrosine-protein kinases.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
