Human DYNLL1 (PIN) Knockout HeLa Cell Lysate

Cat. No.
CLCP-00350523
Product Size
1 Kit

Product Overview

Parental Cell Line
HeLa
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Cervix
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
DYNLL1
Gene ID
UniProt No.
Gene Function
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
Sequence Similarities
Belongs to the dynein light chain family.
Post-translational Modifications
Phosphorylation at Ser-88 appears to control the dimer-monomer transition. It is phosphorylated at Ser-88 by PAK1, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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