Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, homozygous: 5 bp deletion in exon 1.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Gene Function
May play a role in cell proliferation, differentiation and death.
Sequence Similarities
Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.
Post-translational Modifications
Polyubiquitinated; which induces proteolytic degradation and is impaired by interaction with ZC3H15.Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
