Human DNAH5 Knockout A549 Cell Lysate

Cat. No.
CLCP-00350520
Product Size
1 Kit

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 20 and 8 bp deletion in exon 20.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Gender
Male
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
DNAH5
Gene ID
UniProt No.
Gene Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.
Sequence Similarities
Belongs to the dynein heavy chain family.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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