Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 20 and 8 bp deletion in exon 20
Knockout Validation
Sanger Sequencing
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.
Sequence Similarities
Belongs to the dynein heavy chain family.
Storage & Handling
Recommended Control
Human wild-type A549 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
