Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 7 bp deletion in exon 4.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Gene Function
May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
Sequence Similarities
Contains 1 PH domain. Contains 1 START domain.
Post-translational Modifications
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
