Human CDKN1A Knockout HCT116 Cell Lysate

Cat. No.
CLCP-00350503
Product Size
1 Kit

Product Overview

Parental Cell Line
HCT116
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, homozygous: 1 bp insertion in exon 2.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Colon carcinoma
Morphology
Epithelial
Gender
Male
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
CDKN1A
Gene ID
UniProt No.
Gene Function
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Sequence Similarities
Belongs to the CDI family.
Post-translational Modifications
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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