Human CDK5 Knockout HCT116 Cell Line

Inquiry

Cat. No.

CLCP-00350074

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HCT116

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue

Colon carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

CDK5

Gene ID

1020

UniProt No.

Q00535

Gene Function

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Sequence Similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.

Storage & Handling

Recommended Control

Human wild-type HCT116 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

McCoY5a + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.