Human CDK4 Overexpression HEK293T Lysate (whole cell)

Cat. No.
CLCP-00350706
Product Size
1 Kit

Product Overview

Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Species
Human
Knockout Validation
Sanger Sequencing
Tested Applications
WB

Cell Line Properties

Tissue
Kidney
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
CDK4
Gene ID
UniProt No.
Gene Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Shipped on dry ice. Upon delivery aliquot and store at -20 °C. Avoid freeze / thaw cycles.
Storage Buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)

For Research Use Only. Not For Clinical Use.

Online Inquiry