Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Gene Function
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation, when overexpressed in fibroblasts.
Sequence Similarities
Belongs to the BORG/CEP family. Contains 1 CRIB domain.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
