Human BRAF Knockout HCT116 Cell Line

Inquiry

Cat. No.

CLCP-00350031

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HCT116

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue

Colon carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

BRAF

Gene ID

673

UniProt No.

P15056

Gene Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Sequence Similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD (Ras-binding) domain.

Storage & Handling

Recommended Control

Human wild-type HCT116 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

McCoY5a + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.