Human ARPP19 Knockout HEK293T Cell Line

Cat. No.
CLCP-00350021
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Kidney
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Female

Target Information

Gene Name
ARPP19
Gene ID
UniProt No.
Gene Function
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. May indirectly enhance GAP-43 expression.
Sequence Similarities
Belongs to the endosulfine family.
Post-translational Modifications
Phosphorylation at Ser-62 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA. Isoform ARPP-16 contains a N-acetylmethionine at position 1.

Storage & Handling

Recommended Control
Human wild-type HEK293T cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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