Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Sequence Similarities
Belongs to the ANP32 family.Contains 4 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.
Post-translational Modifications
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Storage & Handling
Recommended Control
Human wild-type HEK293T cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
