Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Tissue
Acute monocytic leukemia
Adherent / Suspension
Suspension
Target Information
Gene Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
Sequence Similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.Contains 1 GS domain.Contains 1 protein kinase domain.
Post-translational Modifications
Autophosphorylated.
Storage & Handling
Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
