Human ACTA2 Knockout HCT116 Cell Line

Inquiry

Cat. No.

CLCP-00350003

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HCT116

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue

Colon carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

ACTA2

Gene ID

UniProt No.

P62736

Gene Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Sequence Similarities

Belongs to the actin family.

Storage & Handling

Recommended Control

Human wild-type HCT116 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

McCoY5a + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.