Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
HEK293
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Cell Line Properties
Adherent / Suspension
Adherent
Target Information
Gene Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
Sequence Similarities
Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Storage & Handling
Recommended Control
Human wild-type HEK293 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
