Product Overview
Applications
IHC-P, IP, WB
Target Information
Alternative Names
2700091H24Rik; C88295; FLJ14813; GREATWALL; Greatwall protein kinase; GW; GWL; GWL_HUMAN; hGWL; MAST-L; Mastl; MGC117975; Microtubule associated serine/threonine kinase like; Microtubule-associated serine/threonine-protein kinase-like; RP11 85G18.2; Serine/threonine-protein kinase greatwall; THC2
Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Upon delivery aliquot and store at -20 °C. Avoid freeze / thaw cycles.
Storage Buffer
Tris citrate/phosphate. pH: 7.
Preservative
0.09% Sodium Azide
