Product Overview
Reactivity
Human (predicted: Mouse, Rat)
Target Information
Alternative Names
CUL 3; Cul-3; cul3; CUL3_HUMAN; Cullin 3; Cullin-3; Cullin3; KIAA0617; PHA2E
Function
Core component of multiple cullin-RING-based BCR E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 thus involved in regulation of G1/S transition.
Tissue Specificity
Widely expressed.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Store at 4 °C short term (1-2 weeks). Upon delivery aliquot. Store at -20 °C long term.
Storage Buffer
0.01% Sodium Azide. pH: 6.
Preservative
0.01% Sodium Azide
