Product Overview
Applications
ELISA, IHC-P, WB
Target Information
Alternative Names
Charged multivesicular body protein 4b; CHM4B_HUMAN; chmp4b; Chromatin-modifying protein 4b; hSnf7-2; hVps32-2; SNF7 homolog associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2
Function
Probable core component of the endosomal sorting required for transport complex III which is involved in multivesicular bodies formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6-and p9-dependent virus release.
Tissue Specificity
Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Upon delivery aliquot and store at -20 °C. Avoid repeated freeze / thaw cycles.
Storage Buffer
0.75% Glycine, 1.21% Tris, 2% Sucrose.
