Centrosome Quantification Service

Centrosome abnormalities (CAs) are emerging hallmarks of malignant tumor. The numeric abnormality, known as centrosome amplification, has been reported in variety of cancers. CD BioSciences provides centrosome quantification service with our advanced technology and experienced experts for meeting our global customer's demands in centrosome research and the therapeutic development of centrosome-related disease.

Overview of Centrosome Quantity

The centrosome is the major microtubule-organizing center (MOTC) in cells, who plays important role in defining mitotic spindle poles and forming cilia. Centrosome consists of a pair of centrioles and the surrounding layers of pericentriolar material (PCM). The centrosome copy number is strictly controlled due to its significance of cell cycle. Centrosome numerical alterations cause chromosome missegregation and result in the formation of micronuclei and aneuploid daughter cells. The centrosome abnormalities are frequently observed in cancers, including bladder, blood, bone and soft tissue, brain, breast, cervix, colorectal, head and neck, hepatobiliary tract, kidney, ovary, prostate, and hematological malignancies.

What's more, induction of centrosome abnormalities initiates tumor formation and metastasis in flies. The centrosome abnormalities are not only the bystanders but rather drivers of tumor development and progression. Therefore, due to the association between CAs and tumor classification, transformation and formation, the centrosome abnormalities are considered as potential target for cancer therapy.

Methods of Centrosome Quantification

The copy number of centrosome of tissue samples can be quantified with the cell border marker and the centrosome maker. The selection of centrosome and cell border markers is flexible under specific condition and biological question. And the limitations include high-resolution microscopy and automated quantification program. During the mitosis of cancer cells, the centrosome amplification is frequently observed. Therefore, centrosome quantification is very valuable for identifying tumor subtypes and exploring the promising target for tumor therapy.

Centrosome duplication during cell cycle (Wang M, et al., 2019)

Our Service

For assisting our global customers making better progress in the field of centrosome research, CD BioSciences offers centrosome quantification service in tissues and organs including but not limited as follows.

• Identifying inter- and intratumor heterogeneity.
• Identifying different types of centrosome abnormalities exist in cancer (amplification or loss).
• Tracing the change of centrosome abnormalities during cancer evolution.
• Identifying different centrosome abnormalities predominate in specific cancer types.

Workflow of Our Service

The workflow of our centrosome quantification service is as below.

• Tissue Fixing: normal biopsy specimens, surgical specimens, or tumor microarrays.
• Tissue Staining: centrosomal protein, such as CEP135, CENT3, CP110 and PCM proteins, are targeted for identifying centrosomes, while the cell border will be identified with proteins like E-cadherin and α6-integrin.
• Imaging: Immunofluorescence microscopy is used for imaging specific area.
• Analysis and Centrosome Quantification: images are compiled for identifying the centrosome and cell border.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

1. Wang M, et al., A method of quantifying centrosomes at the single-cell level in human normal and cancer tissue. Mol Biol Cell. 2019 Mar 21;30(7):811-819.

Our services are for research use only and not for any clinical use.