Centrosome Localization Service

The centrosome is a major organizer of microtubules that has important functions in regulating cell shape, polarity, cilia formation and intracellular transport as well as the position of cellular structures, including the mitotic spindle. CD BioSciences provides centrosome localization service with our advanced technology and experienced experts for meeting our global customer's demands in centrosome research and the therapeutic development of centrosome-related disease.

Overview of Centrosome Localization

Centrosome is the major microtubule organizing centers (MTOCs) in most cells. Microtubules (MTs) are important cytoskeletal structures, essential for cell division, intracellular transport, motion of cilia and flagella, as well as cell migration and establishment of polarity. During the mitosis, centrosome duplicate once at the beginning of G1 phase and each of them is localized in each pole of the spindle for maintaining the cell bipolarity. By these activities, centrosome is involved regulating polarized cell behaviors, such as cell migration or neurite outgrowth, as well as mitotic spindle orientation.

Localization of Centrosomes in Cell Migration

In slow-moving cell, the centrosome is usually located in front of the nucleus (relative to the direction of cell migration) and MTs radiate primarily towards the leading edge. In fast-moving cell, centrosome and MTs localize behind the nucleus during directional migration.

Fibroblasts

In non-polarized fibroblasts (left), the centrosome is located near the center of the cell and is physically linked to the nucleus. During wound healing, the fibroblast centrosome often becomes oriented between the nucleus and the leading edge.

Epithelial cells

In polarized epithelial cells (left), centrosomes are located on the apical surface of the cell. In multiciliated epithelial cells (right), hundreds of centrioles are assembled at once in a single cell, leading to the formation of multiple cilia.

Neurons

In resting neurons (left), the centrosome is found in close proximity to the neurite that becomes the axon. In migrating neurons (right), the centrosome is positioned ahead of the nucleus, with microtubules connecting the centrosome and the nucleus.

Lymphocytes

The centrosome directs the delivery of lytic granules by moving along microtubules to the plasma membrane and then to the point of secretion for releasing lytic granules to the immunological synapse after the CTL is stimulated.

Methods for Centrosome Localization

Live-cell imaging is an important analytical tool in biological research, which provides the subcellular events and biological processes. With the variety of fluorescent proteins and multicolored probes that have been developed, it is now possible to label virtually any molecule. Centrosome is localized by placing genetically encoded fluorescent protein (FP) tags at the N- or C-termini of centrosome proteins, such as tubulin, PCM proteins or CEP proteins.

Applications of Centrosome Localization Detection

• Studying cyto-dynamics and cell migration.
• Studying cell cycle
• Revealing the relevance between the migration of cancer cells and centrosome localization

Our Service

For assisting our global customers making better progress in the field of centrosome research, CD BioSciences offers centrosome localization detection service based on technologies including but not limited as follows.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Our services are for research use only and not for any clinical use.