Centrosome Isolation Service

Centrosome is the major microtubule organizing centers (MTOCs) in most cells. For launching further research of centrosome, such as component detection, mRNA-seq or protein MS, high quality centrosome isolation is necessary. CD BioSciences provides centrosome quantification service with our advanced technology and experienced experts for meeting our global customer's demands in centrosome research and the therapeutic development of centrosome-related disease.

Overview of Centrosome Isolation

Centrosome is a cytoplasmic organelle plays important role in many biological processes, such as mitosis, assembling spindle and cell division, as well as cell migration, invasion and polarity. Centrosome is consisted of a pair of cylindrical structures known as centrioles, decorated at their distal ends by appendage structures, and surrounded by a proteinaceous matrix known as the pericentriolar material (PCM). Due to the high-association between centrosome abnormality and malignant tumor, it's valuable to study the spatial and temporal dynamics of centrosome composition and its variation between different cells and tissue.

Methods of Centrosome Isolation

Glycerol Cushion Sedimentation

Animal tissues, culture animal cells, or plant tissue are harvested. After preparation of a cytoplasmic extract, Taxol was added to induce microtubule polymerization. Microtubules and centrosomal mRNAs were purified by sedimentation through a glycerol cushion.

Sucrose Density Gradient Centrifugation

To isolate pure centrosome from cultured cells or animal tissues, sucrose gradient centrifugation will be conducted. A low ionic strength buffer can help centrosome detach from nuclei and intermediate filaments. After aggregation and centrifugation, chromatin can be removed, and centrosomes are sedimented onto a sucrose cushion and further enriched by sucrose gradient centrifugation.

Affinity Capture

This method involves a single-step affinity purification of centrosomes by selecting proteins known to localize to distinct sites of the centrosome. And they are immunoprecipitated from induced cells, and reproducibly determined their interactome with mass spectrometry.

Applications of Centrosome Isolation

• Analysis proteomic composition of centrosome.
• RNA-seq of centrosome.
• Identifying uncharacterized centrosomal proteins.
• Revealing the relevance between centrosome component and disease.

Our Service

For assisting our global customers making better progress in centrosome research, CD BioSciences offers centrosome isolation services in cells and tissues including but not limited as follows.

• Human tissue: tumor tissues, nerve cells, and other tissue of pathological changes.
• Mouse tissue: early embryos, spermatogonium, oocytes, tumor tissue, nerve cells, and other tissues and cells.
• Cell lines: Hela cells, 3T3 cells, A549 cells, HEK 293 cells, Jurkat cells, OK cells, Ptk2 cells, Vero cells and others.
• Xenopus laevis: early embryos, spermatogonium, oocytes, eggs, and many other tissues and cells.
• Drosophila: early embryos, tissues and individuals.
• Caenorhabditis elegans: embryos and individuals.
• Plant tissue: leaves, stem, root, flower, pollen, pistil, and cultured plant tissues.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Our services are for research use only and not for any clinical use.