Centrosomal Protein Structure Determination Service

Centrosomal proteins the main constituent of the centrosome. Accurate the understanding of 3D structure of centrosomal proteins by structure prediction and assessment can facilitate the study of their biological functions and the development of related applications. CD BioSciences offers high-quality and professional centrosomal protein prediction and assessment service for research use. With advanced technology, equipment, and experienced scientists, we have assisted our global customers in the field of centrosome research.

Overview of Centrosomal Protein Structure

Centrosome is a cell organelle that is constituted by two centrioles, highly structured macromolecular complexes typically consisting of nine microtubule-triplet-blades arranged in a cylinder, which also form the basal bodies required for the formation of cilia and flagella. pericentriolar material (PCM) are necessary during the biological processes of centrosome, which is a protein matrix that are main constituent of centrosome.

The centrosomal proteins tend to be larger than generic proteins and relatively rich in predicted disordered regions. The main characteristics of centrosomal proteins are the numerous disorders and coiled-coil regions that make them extremely flexible and able to interact with many partners, forming intertwined coiled-coils.

The protein structure prediction is the process predicting the secondary, tertiary, and quaternary structures of proteins based on the primary structure or amino acid sequence of proteins. The information of centrosomal protein including protein length, protein folding, protein motif and other traits are provided by protein structure prediction.

Methods of Protein Structure Determination

X-ray crystallography

X-ray crystallography is the technology determining the atomic and molecular structure of a crystal, in which the crystal structure changes the rote of X-rays beam diffract into many specific directions. By mearing the angles and intensities, a three-dimensional picture of the density of electrons within the crystal can be produced and the mean positions of the atoms in the crystal can be determined, as well as crystallographic disorder, chemical bonds, and various other information.

Protocol of X-ray crystallography

NMR spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy of proteins is used to obtain information about the structure and dynamics of proteins. NMR involves the quantum-mechanical properties of the central core of the atom, which depend on the local molecular environment, and their measurement provides a map of space, movement and chemical bound between atoms.

Protocol of NMR spectroscopy (Kaur H, et al. 2019)

Cryo-electron microscopy (Cryo-EM)

The essential mechanism of Cryo-EM is electron scattering. The coherent electrons are used as a light source to measure the sample. After the electron beam passes through the sample and the nearby ice layer, the lens system converts the scattered signal into a magnified image recorded on the detector. And signal processing is performed to obtain the three-dimensional structure of the sample. With our cryo-EM service, the high-resolution biological structure of centrosomal protein can be determined.

Protocol of Cryo-EM (Carroni M, et al. 2016)

Our Services

With advanced equipment and mature technology, CD BioSciences offer protein structure detection services including but not limited as follows.

Technologies	Advantages	Object
X-ray crystallography	Well developed High resolution Broad molecular weight range Easy for model building	Crystallizable samples Soluble proteins
NMR spectroscopy	High resolution 3D structure in solution Good for dynamic study	MWs below 40–50 kDa Water soluble samples
Cryo-EM	Easy sample preparation Structure in native state Small sample size	>150 kDa Complex compounds

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

References

1. Kaur H, et al. Analytical techniques for characterization of biological molecules - proteins and aptamers/oligonucleotides. Bioanalysis. 2019 Jan;11(2):103-117.
2. Carroni M, et al. Cryo electron microscopy to determine the structure of macromolecular complexes. Methods. 2016 Feb 15;95:78-85.

Our services are for research use only and not for any clinical use.