Centrosomal Protein PTMs Analyzing Service

Post-translational modifications (PTMs) affect the structure, stability, functions and PPIs of centrosomal proteins. The definition and characterization of PTMs are very important in the discovery of the mechanisms of many biological processes. CD BioSciences offers high-quality and professional centrosomal protein PTMs analyzing service for research use. With advanced technology, equipment, and experienced scientists, we have assisted our global customers in the field of centrosome research.

PTMs of Centrosomal Proteins

Centrosome is the main microtubule organizing center (MTOC) of most cells. And most microtubules involved in mitosis are generated from centrosome. The microtubule is formed by thirteen laterally associated protofilaments of α/β-tubulin heterodimers, which are encoded by several genes that varies in their amino acid composition and C-terminal tail regarding length. In addition, α- and β-tubulin isotypes may undergo multiple post-translational modifications (PTMs), including detyrosination, acetylation, polyglutamylation, polyglycylation, phosphorylation, ubiquitination, sumoylation, and palmitoylation.

PTMs in centrosomal proteins (Lopes D, et al. 2020)

In addition, the PTMs of tubulin are considered as potential biomarkers of cancers, as well as for therapeutic purposes. It has been observed that PTMs occur along with specific cancer properties, such as poor outcome/prognosis and metastatic ability.

The mass spectrometry (MS) can not only identify the protein, the modification types, but also locating the modifications sites without previous knowledge. Therefore, MS is widely used for protein modifications in biopharmaceutical industry for quality control and lot release.

Bottom-up (BU) and top-down (TD) are the two complementary approaches in MS-based proteomics. The BU-MS involves proteolytic digestion of proteins before analysis, while the TD-MS is a powerful technology for characterization of protein modifications which preserved the structure of proteins.

Our Services

As the main way to regulate cellular processes, PTMs are very tightly modulated and generally occur either transiently and/or at very low levels. CD BioSciences provides PTMs identification, characterization, and quantitative analysis of centrosomal proteins, with the high-resolution MS and professional bioinformatics analysis. The PTMs types our service covers from the samples are including but not limited as follows.

Workflow of Centrosomal PTM Analyzing Service

The results of our PTMs analysis service contains:

• Basic statistics: average, standard deviation and %CV and fold change for group comparisons in a peptide-centric manner
• Significance assessing: confidence interval or a Student's T test
• Bioinformatics analysis: Principal Component Analysis (PCA), heatmap clustering, gene ontology (GO) and any other analysis as customer requires.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

1. Lopes D, Maiato H. The Tubulin Code in Mitosis and Cancer. Cells. 2020 Oct 26;9(11):2356. doi: 10.3390/cells9112356. PMID: 33114575; PMCID: PMC7692294.

Our services are for research use only and not for any clinical use.