Centrosomal Protein Isolation Service
InquiryCentrosome is the cell organelle formed from proteins and mRNAs. During the study of centrosomal protein, its isolation is the first and the key step. A high-quality protein isolation result is the basis of following analysis. CD BioSciences offers high-quality and professional centrosomal protein isolation and purification service from animal tissues and cultured animal cells for research use. With advanced technology, equipment, and experienced scientists, we have assisted our global customers in the field of centrosome research.
Introduction into Centrosomal Protein
During the cell cycle, the structure, function and quantity of centrosome are tightly restricted. The proper duplication, maturation, and separation of centrosome are the basis of bipolar spindle formation, which is assurance of chromosome segregation and cell division. Multiple proteins are involved in centrosome duplication. For example, during the exit of mitosis, a well-established protease facilitates disengagement of centriole in the cleavage of sister chromatids in the anaphase. Moreover, several structural proteins, such as PCM and centriolar components, are essential for the assembly of procentriole. Both of the maturity and separation need enrichment of extra PMCs and activity of kinases. Here is the list of few centrosomal proteins.
Methods of Protein Isolation
Affinity Chromatography
Affinity Chromatography is a separation technique based upon molecular conformation that can specifically isolate analytes. Immunoaffinity chromatography is based on the specific binding of antibody- antigen. And the affinity tags can be cleaved off by a protease.
Ion Exchange Chromatography
Ion exchange chromatography is the protein purification technology based on the nature and degree of analytes' ionic charge. It's frequently used for analytical and preparative separations of centrosomal proteins.
Size Exclusion Chromatography
Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is the protein purification technology the size or hydrodynamic volume (radius of gyration) of the analytes. With SEC, the weights and distribution of centrosomal protein molecular can be easily estimated.
Free-flow-electrophoresis
Free-flow electrophoresis (FFE) is a carrier-free electrophoresis technique, which allows to separate protein isoforms. It's a very powerful tool for isolating centrosomal proteins with separate isoforms.
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography (HIC) is a protein purification technology based on the surface hydrophobicity of analyte. Target proteins and their product aggregate species tend to have different hydrophobic properties, and HIC can be used for further purifies the centrosomal protein of interest.
High Performance Liquid Chromatography
High performance liquid chromatography (HPLC) is a technology used to separate, identify, and quantify each component in a mixture.
Steps of affinity chromatography (Urh, et al. 2009)
Our Service
CD BioSciences established mature platform for centrosomal protein isolation and purification, offering reliable services for our global customers. With our advanced technology and experienced sciences, we provide optimal strategies of centrosomal protein isolation and purification services, for both single protein and multiple proteins, small-scale purification and large-scale purification.
Technologies of Our Protein Isolation Service:
- Affinity Chromatography
- Ion Exchange Chromatography
- Size Exclusion Chromatography
- Free-flow-electrophoresis
- Hydrophobic Interaction Chromatography
- High Performance Liquid Chromatography
Why Choose Us
Professional Team
Comprehensive Platform
High Efficiency
One-stop Service
With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.
Reference
- Urh, et al. "Affinity chromatography: general methods." Methods in enzymology 463 (2009): 417-438.
Our services are for research use only and not for any clinical use.
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